黃素腺嘌呤二核苷酸檢測(cè)試劑盒
產(chǎn)品名稱：黃素腺嘌呤二核苷酸檢測(cè)試劑盒
英文名稱：FAD Assay Kit
貨號(hào)：K357-100
品牌：原裝進(jìn)口
包裝：100次分析
保存建議: 請(qǐng)?jiān)诮邮盏皆?/font>黃素腺嘌呤二核苷酸檢測(cè)試劑盒產(chǎn)品后，置于+4℃保存.
產(chǎn)品背景: 黃素腺嘌呤二核苷酸（FAD）是氧化還原反應(yīng)的輔因子，在代謝中起重要作用.FAD在不同的氧化還原態(tài)以FAD， FADH或FADH2的形式存在和循環(huán).真核代謝中還原態(tài)FAD的來(lái)源是三羧酸循環(huán)和β氧化途徑.
產(chǎn)品描述: FAD檢測(cè)試劑盒中，F(xiàn)AD作為一個(gè)氧化酶的輔因子催化產(chǎn)物的形成，該產(chǎn)物與OxiRed探針反應(yīng)，顯色（λmax = 570 nm）和發(fā)出熒光（Ex/Em =535/587 nm）.試劑盒快速、簡(jiǎn)單、超敏、結(jié)果可靠，適于高通量分析.檢出限低于1 nM.
產(chǎn)品特點(diǎn): 1、步驟簡(jiǎn)單；2、操作快速并且方便；3、分析靈敏、穩(wěn)定并且適合高通量分析
1. Standard Curve Preparation:
For the colorimetric assay, dilute the 10 pmol/μl FAD Standard solution to 0.2 pmol/μl by
adding 10 μlof FAD Standard to 490 μl of Assay Buffer, mix well. Add 0, 2, 4, 6, 8, 10 μl into a
series of wells. Adjust volume to 50 μl/well with Assay Buffer to generate 0, 0.4, 0.8, 1.2, 1.6, 2
pmol/well of the FAD Standard. Use a freshly diluted Standard each time.
For the fluorometric assay, dilute the FAD Standard solution to 0.02 pmol/μl by adding 10 μl of
the 10 pmol/μl FAD Standard to 490 μl of Assay Buffer, mix well. Then take 10 μl to 90 μll of
Assay Buffer. Add 0, 2, 4, 6, 8, 10 μl into a series of wells. Adjust volume to 50 μl/well with
Assay Buffer to generate 0, 0.04, 0.08, 0.12, 0.16, 0.2 pmol/well of the FAD Standard.
2. Sample Preparations:
Homogenize tissue (5 - 20 mg) or cells (1 x 10
6) in 400 μl of Assay Buffer, centrifuge to remove
insoluble material at 13,000 x g for 3 mins. Serum samples should be collected using EDTA to
inhibit enzymes that may degrade FAD. Deproteinize sample using perchloric acid precipitation
(BioVision, Cat.# K808-200, Deproteinizing Sample Prep Kit) to release FAD from proteins. After
deproteinization, add samples directly to 96 well plate, bring the volume to 50 μl/well with Assay
Buffer. Approximate sample amount per assay: ~0.1 - 0.5 mg tissue; ~10,000 - 100,000 cultured
cells, ~0.1 - 20 μl serum. For unknown samples, we suggest testing several doses of your
sample to ensure the readings are within the standard curve range.
如與英文說明書有異，以英文說明書為準(zhǔn)。
黃素腺嘌呤二核苷酸檢測(cè)試劑盒說明書具體咨詢咨詢
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