組織蛋白酶(Cathepsin-H)活性檢測試劑盒說明書
產(chǎn)品名稱:組織蛋白酶(Cathepsin-H)活性檢測試劑盒
英文名稱:Cathepsin H Activity Assay Kit
貨號(hào):K145-100
品牌:原裝進(jìn)口
規(guī)格:100T
保存建議: 請(qǐng)?jiān)诮邮盏?/font>組織蛋白酶(Cathepsin-H)活性檢測試劑盒產(chǎn)品后,置于-20℃保存.
試劑盒簡介: Cathepsin-H活性檢測試劑盒是一種基于熒光的檢測,利用以MCA標(biāo)記Cathepsin-H首選的底物序列GKPILFFRLK(Dnp)-D-R-NH2).細(xì)胞溶解物或者其它樣品中包含的Cathepsin-H酶切這種該底物之后,產(chǎn)物能釋放熒光,通過熒光計(jì)或者熒光微量滴定板讀數(shù)器即可輕易進(jìn)行定量檢測(Ex/Em = 328/460 nm).
產(chǎn)品背景: 除了傳統(tǒng)的Caspase介導(dǎo)的切割小體之外,凋亡還可以通過其他機(jī)制來介導(dǎo).越來越多的證據(jù)表明替代蛋白水解酶譬如溶酶體組織蛋白水解酶有可能其實(shí)或者傳播凋亡信號(hào).組織蛋白酶(Cathepsin)為溶酶體內(nèi)的酶,包括Cathepsin B、Cathepsin K、Cathepsin L 和Cathepsin S等多種類型,參與著細(xì)胞凋亡的非Caspase 途徑,同時(shí)也是多種毒理作用的敏感指標(biāo).
產(chǎn)品特點(diǎn): 1、簡易的一步法技術(shù),1-2小時(shí)即可完成;2、操作方便快捷;3、蛋白酶-H檢測直截了當(dāng),可用于96孔板檢測.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals.Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-H Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-H substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-H will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The cathepsin-H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions
have been optimized to obtain the maximal activity.
如與英文說明書有異,以英文說明書為準(zhǔn)。
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