硫氧還蛋白還原酶檢測試劑盒
產品名稱:硫氧還蛋白還原酶檢測試劑盒
英文名稱:Thioredoxin Reductase Assay Kit
貨號:K763-100
品牌:原裝進口
包裝:100次分析
保存建議: 請在接收到該硫氧還蛋白還原酶檢測試劑盒產品后,置于-20℃保存.
產品背景: 硫氧還蛋白還原酶(Thioredoxin Reductase ,TrxR)是一種NADPH依賴的包含FAD結構域的二聚體硒酶,屬于吡啶核苷酸-二硫化物氧化還原酶家族成員,包含保守的 Cys Val Asn Val Gly Cys 氧化還原位點 ,催化依賴硫氧還蛋白NADPH還原反應.它和硫氧還蛋白、NADPH共同構成了硫氧還蛋白系統(tǒng).TrxR具有多種生物學活性 ,調節(jié)機體氧化還原反應和細胞生長與增殖 ,與人類某些疾病發(fā)病機制有關 ,可能是干預治療的靶點.
產品描述: 硫氧還蛋白還原酶檢測試劑盒提供了一種方便的、高靈敏的比色方法來檢測不同生物學標本中硫氧還蛋白還原酶的活性.在該試劑盒反應里,硫氧還蛋白還原酶 (Thioredoxin Reductase ,TrxR)催化NADPH使DTNB還原成TNB2-,后者產生較強的黃色(λmax = 412 nm).然而,在一般粗提液里也包含其他能還原DTNB的酶類,比如谷胱甘肽還原酶、谷胱甘肽過氧化物酶,因此,TrxR的特異性抑制劑也被用于該分析中.該試劑盒同時提供兩個檢測分析:首先是檢測樣本內DTNB的總還原量,然后是添加了TrxR的特異性抑制劑后樣本內DTNB的總還原量,兩者的差異即是TrxR對DTNB的還原量.
產品特點: 1、步驟簡單,只需要大約30-40分鐘即可完成檢測;2、操作快速并且方便
Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many
cellular processes such as cell growth, p53 activity, and protection against oxidation stress,
etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as
selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. BioVision’s Thioredoxin
Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in
various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic)
acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong
yellow color (λmax = 412 nm). Since in crude biological samples other enzymes, such as
glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR
specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the
first measurement is of the total DTNB reduction by the sample, and the second one is the
DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference
between the two results is the DTNB reduction by TrxR.
如與英文說明書有異,以英文說明書為準。
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