組織蛋白酶D抑制劑篩選熒光試劑盒說(shuō)明書
產(chǎn)品名稱：組織蛋白酶D抑制劑篩選熒光試劑盒
英文名稱：Cathepsin D Inhibitor Screening Kit （Fluorometric）
貨號(hào)：K148-100
分類：原裝進(jìn)口
規(guī)格：100T
保存建議 請(qǐng)?jiān)诮邮盏浇M織蛋白酶D抑制劑篩選熒光試劑盒產(chǎn)品后，置于-20℃保存
英文簡(jiǎn)介： Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals.Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin D substrate sequence GKPILFFRLK(DNP)-D-RNH2)labeled with MCA. Cathepsin D will cleave the synthetic substrate to release the quenched fluorescent group MCA, which can then easily be measured using a fluorometer or fluorescence plate reader at Ex/Em = 320/420 nm. The relative efficacy of test inhibitors are compared to the positive control inhibitor, Pepstatin A (IC50 < 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).
試劑盒中文簡(jiǎn)介: 組織蛋白酶D抑制劑篩選試劑盒利用偶聯(lián)有MCA的組織蛋白酶D經(jīng)典底物GKPILFFRLK（Dnp）-D-R-NH2），當(dāng)此底物被酶切后，熒光基團(tuán)MCA被釋放.利用熒光儀或者熒光酶標(biāo)儀檢測(cè)出熒光活性，Ex/Em = 328/460 nm.抑制劑的相對(duì)抑制效率可與陽(yáng)性對(duì)照，Pepstain A （IC50 < 0.1 nM）做參考.
產(chǎn)品背景: 細(xì)胞凋亡除了被經(jīng)典的Caspase介導(dǎo)的酶切激活外，還能別其他機(jī)制激活.越來(lái)越多的人發(fā)現(xiàn)另外一種蛋白質(zhì)水解酶，溶酶體的組織蛋白酶水解酶也可以起始和促進(jìn)凋亡信號(hào).組織蛋白酶作為一種溶酶體的酶，在不同的毒性研究中，也被作為靈敏的標(biāo)志物.
產(chǎn)品特點(diǎn): 1、簡(jiǎn)單的一步式操作，1小時(shí)完成實(shí)驗(yàn).2、快速，明顯，便捷.3、快速高效的篩選潛在的抑制劑.4、試劑盒提供，陽(yáng)性對(duì)照抑制劑，Pepstatin A.
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