谷胱甘肽(氧化型/還原型/總量)檢測試劑盒
產(chǎn)品名稱:谷胱甘肽(氧化型/還原型/總量)檢測試劑盒
英文名稱:Glutathione (GSH/GSSG/Total) Assay Kit
貨號:K264-100
品牌:原裝進(jìn)口
包裝:100次分析
保存建議: 請?jiān)诮邮盏皆?/font>Biovision品牌的谷胱甘肽(氧化型/還原型/總量)檢測試劑盒產(chǎn)品后,置于-20℃保存.
產(chǎn)品背景: 谷胱甘肽是哺乳動物細(xì)胞中抗氧化脅迫最重要的低分子硫醇.在凋亡早期能觀測到谷胱甘肽的降解.還原型谷胱甘肽(GSH)是人體組織中用于清除反應(yīng)性氧(ROS)的抗氧化劑,氧化型谷胱甘肽(GSSG)在谷胱甘肽還原酶(GR)的作用下可還原成GSH.許多細(xì)胞發(fā)生凋亡時,細(xì)胞漿內(nèi)的GSH水平會降低,此現(xiàn)象發(fā)生在細(xì)胞凋亡非常早的階段,因此檢測谷胱甘肽的水平可以早期判斷和檢測凋亡.商業(yè)的谷胱甘肽檢測試劑盒,比如DTNB-酶循環(huán)谷胱甘肽比色分析試劑盒或者基于Monochlorobimane(MCB)熒光分析試劑很難區(qū)分GSH(FW:307)和GSSG(FW:612).
產(chǎn)品描述: 谷胱甘肽檢測試劑盒提供了特有的,方便的工具來分別檢測還原型GSH,氧化型GSSG和總谷胱甘肽.在本谷胱甘肽檢測試劑盒中,OPA與還原型GSH反應(yīng)(不與氧化型GSSG反應(yīng)),產(chǎn)生熒光.因此GSH可以被特異地檢測.加上還原試劑后,氧化型GSSG轉(zhuǎn)化成還原型GSH,因此GSH+GSSG可以被檢測.而如果只需要檢測氧化型GSSG,先加入試劑盒內(nèi)提供的GSH淬滅劑,阻止其與0PA反應(yīng),而GSSG則不受影響,這樣可特異性的檢測氧化型GSSG.試劑盒提供獨(dú)特的技術(shù)和緩沖液配方消除其它蛋白巰基的影響,并在操作過程中穩(wěn)定GSH和GSSG.
產(chǎn)品特點(diǎn): 1、步驟簡單,只需要大約1-2小時即可完成檢測;2、操作快速并且方便; 3、簡單靈敏,可檢測2-400 ng/l濃度的GSH、GSSG或總谷胱甘肽
Glutathione is the major intracellular low-molecular-weight thiol that plays a critical role in
cellular defense against oxidative stress in tissues and cells. Commercially available
glutathione detection kits, such as the DTNB-enzyme cycling glutathione assay kit or the
Monochlorobimane based assay kit hardly distinguish between reduced glutathione (GSH; FW:
307) and oxidized glutathione (GSSG; FW: 612). BioVision’s Glutathione Detection Kit provides
a unique, convenient tool for detecting GSH, GSSG, and total glutathione separately. In the
assay, OPA, reacts with GSH (not GSSG), generating fluorescence, so GSH can be
specifically quantified. Adding a reducing agent converts GSSG to GSH, so (GSH + GSSG)
can be determined. To measure GSSG specifically, a GSH Quencher is added to remove
GSH, preventing reaction with OPA (while GSSG is unaffected). Reducing agent is then added
to destroy excess quencher and convert GSSG to GSH. Thus, GSSG can be specifically
quantified. The kit provides a unique procedure and buffer formula to eliminate protein thiol
interference and to stabilize GSH and GSSG in solution. The assay is easy to perform and
detects 2-400 ng/μl of GSH, GSSG or total glutathione
如與英文說明書有異,以英文說明書為準(zhǔn)。
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