葡萄糖-6-磷酸(PicoProbe)檢測試劑盒說明書
產(chǎn)品名稱:葡萄糖-6-磷酸(PicoProbe)檢測試劑盒
英文名稱:PicoProbe Glucose-6-Phosphate Assay Kit
貨號:K687-100
分類:原裝進口
規(guī)格:100T
英文簡介:Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 μM in the original sample assuming a dilution of 5X during processing. The PicoProbeTM G6P Assay Kit is much more sensitive than the G6P Colorimetric Assay Kit for low G6P samples.
試劑盒中文簡介:葡萄糖6磷酸分析試劑盒能簡單,靈敏,快速的定量多種生物樣本中的G6P.在實驗過程中,G6P被氧化,其產(chǎn)物能把近無色的探針轉(zhuǎn)變成高熒光強度產(chǎn)物(Ex/Em 535/587 nm).本試劑盒的檢測范圍10 -500 pmoles等價于濃度在范圍1-500uM初始樣本在處理期間稀釋5次.本試劑盒比G6P比色法分析試劑盒更靈敏,適應(yīng)于低G6P含量的樣本.
產(chǎn)品背景: 葡萄糖-6磷酸酶是葡萄糖進入細胞然后進入代謝途徑或者儲存過程中的關(guān)鍵中間體.G6P能進入糖酵解途徑,戊糖旁路或者糖原或者淀粉的合成.G6P通過的它的脫氫酶產(chǎn)生還原當量NAD(P)H.G6PDH在紅細胞中尤其重要,缺乏它就會引起溶血性貧血.
產(chǎn)品特點: 1、簡單操作,35-40分鐘就可以完成實驗;2、快速方便;3、靈敏,高效,精確.
保存條件:請在接收到葡萄糖-6-磷酸(PicoProbe)檢測試劑盒后,置于-20℃保存.
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