FRASC抗壞血酸（維生素C）檢測(cè)試劑盒說(shuō)明書
產(chǎn)品名稱：FRASC抗壞血酸（維生素C）檢測(cè)試劑盒
英文名稱：Ascorbic Acid Assay Kit
貨號(hào)：K671-100
品牌：原裝進(jìn)口
規(guī)格：100T
保存建議: 請(qǐng)?jiān)诮邮盏?/font>FRASC抗壞血酸（維生素C）檢測(cè)試劑盒產(chǎn)品后，置于-20℃保存.
試劑盒簡(jiǎn)介: 抗壞血酸（維生素C）檢測(cè)試劑盒能快速靈敏地檢測(cè)各種生物樣品中（比如血清和其他體液、組織和細(xì)胞抽提液、生長(zhǎng)培養(yǎng)基和食品）抗壞血酸的含量，操作簡(jiǎn)單.檢測(cè)實(shí)驗(yàn)中，F(xiàn)e（3+）離子會(huì)被樣品中所有的抗氧化劑還原成Fe（2+）.后者能螯合探針，產(chǎn)物在545-600 nm有強(qiáng)吸收譜.在平行實(shí)驗(yàn)中加入抗壞血酸氧化酶能去除抗壞血酸產(chǎn)生的吸光度，測(cè)量出背景吸收值.總吸收值減去背景吸收值就能得到抗壞血酸的含量.檢測(cè)量程為0.2 - 20 nmol
產(chǎn)品背景: 抗壞血酸（Ascorbic Acid）又叫維生素C（Vitamin C），是一種水溶性維生素.正常成人體內(nèi)的抗壞血酸代謝活性池中約有1500mg抗壞血酸，最高儲(chǔ)存峰值為3000mg抗壞血酸.抗壞血酸在氧化還原代謝反應(yīng)中起調(diào)節(jié)作用，缺乏它可引起壞血病.抗壞血酸是一種強(qiáng)力的抗氧化劑、抗病毒和抗炎劑.廣泛存在于食品和生物樣本中，是一種免疫刺激劑.因此檢測(cè)抗壞血酸在上述樣本內(nèi)的分布和含量就顯得非常重要.然而由于血清等樣品中往往還存在其它抗氧化劑，絕大數(shù)抗壞血酸檢測(cè)技術(shù)受強(qiáng)烈的干擾，導(dǎo)致結(jié)果不準(zhǔn)確.
產(chǎn)品特點(diǎn): 1、步驟簡(jiǎn)單，只需30分鐘；2、操作快速并且方便
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. BioVision’s FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids,
tissue and cell extracts, growth media and food products. In this assay, Fe+3 is reduced to Fe+2 by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545 -600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
如與英文說(shuō)明書有異，以英文說(shuō)明書為準(zhǔn)。
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